Diversity of methicillin-resistant coagulase-negative Staphylococcus spp. and methicillin-resistant Mammaliicoccus spp. isolated from ruminants and New World camelids

Information about livestock carrying methicillin-resistant coagulase-negative staphylococci and mammaliicocci (MRCoNS/MRM) is scarce. The study was designed to gain knowledge of the prevalence, phenotypic genotypic antimicrobial resistance genetic diversity MRCoNS/MRM originating from ruminants New World camelids. In addition, a multi-locus sequence typing scheme for characterization Mammaliicoccus (formerly Staphylococcus) sciuri developed. conducted April 2014 January 2017 at University Clinic Ruminants Institute Microbiology Veterinary Medicine Vienna. Seven hundred twenty-three nasal swabs camelids with without clinical signs were examined. After isolation, identified by MALDI-TOF, rpoB sequencing typed DNA microarray-based analysis PCR. Antimicrobial susceptibility testing agar disk diffusion. From all 723 swabs, 189 obtained. Members (M.) group predominant (M. (n = 130), followed M. lentus 43), fleurettii 11)). total, 158 out isolates showed phenotypically multi-resistance profile. A seven-loci includes internal segments house-keeping genes ack, aroE, ftsZ, glpK, gmk, pta1 tpiA. 28 different types (STs) among 92 selected isolates. ST1 most prevalent ST 35), 2 15), ST3 ST5 (each n 5), ST4 3), ST6, ST7, ST8, ST9, ST10 ST11 2).